ABSTRACT
For intensive rearing of fish with minimal losses, it is necessary to be aware of health status of fish. Blood variables are useful criteria for showing physiological disturbances in intensively farmed fishes and can provide important information for diagnosis and prognosis of diseases. The aim of the present study was to show hematological parameters in haematological evaluation is gradually becoming a routine practice for Shirbout fish when intensively bred. In this study, 60 clinically healthy Barbus grypus, were caught from culturing pools in Khuzestan province. Blood samples were taken from caudal vein and the levels of hematological parameters were determined. Comparison between sexes were done using student t-test and Pearson correlation coefficient with Fisher's test. In this study the overall mean of total red blood cells, total leukocyte count, hemoglobin, mean cell volume [MCV], mean cell hemoglobin [MCH], mean cell hemoglobin concentration [MCHC], hematocrit, lymphocyte, neutrophil, eosinophil, and monocyte were [1.41 +/- 0.035][x]10[6], [12.5+.-0.57][x]10[3] cell/1, 6.5 +/- 0.1 g/dl, 261 +/- 4.87fl, 45.7 +/- 0.88pg, 17.6 +/- 0.27, 36.9 +/- 0.7, 56.2 +/- 0.59, 40 +/- 0.52, 1.1 +/- 0.15 and 2.8 +/- 0.22%, respectively. In terms of these parameters, there was not significant difference between sexes
Subject(s)
Animals , Sex , Hematologic Tests , FishesABSTRACT
Macrocysts of S. fusiformis were teased out from the oesophageal muscles of infected buffaloes and a suspension of cyst extract with protein concentration of 2mg/ml was used as uncooked [UE] and cooked [CE] form. Thirty six healthy male laboratory rabbits were divided into six equal groups randomly. Group I and II were inoculated with UE, group III and IVwere inoculated with CE and group Vand VI were inoculated with PBS orally and intraperitonealy [1 ml/kg] respectively. Blood samples were taken in 0, 3, 6, 24 hrs, 3 and 7 days after inoculation. Coagulation tests including BT, CT, PT, APTT, fibrinogen level and platelet count were measured at the different times which mentioned in all groups. Statistical analysis showed significant differences between different times of all tests in group II compare to other groups [p < 0.05]. The study showed that IP inoculation of UE of S. fusiformis at protein concentration of 2mg/ml is lethal to rabbits about 24 hr after inoculation, and caused significant changes in blood coagulation tests in different times. The results also showed that S.fusiformis extract toxin was thermolabile as it was inactivated at 60 ?Cfor 30 min. In addition oral administration of the cysts extract did not make any changes in both cooked and uncooked form. Thus it seems that orally inoculation of S. fusiformis cysts extract had no toxic effects on coagulation tests mentioned above in rabbits because the toxin is probably susceptible to the gastric enzymes and low pH of the stomach
Subject(s)
Animals , Blood Coagulation , Blood Coagulation Tests , Administration, Oral , RabbitsABSTRACT
In order to determine the lipids and lipoproteins profiles in Iranian khouzestanian Arab horses, blood samples were collected from the jugular vein of 77 horses[47 female, 30 male] from different age groups[< 6, 7-12, 12-36, 37-60, 61-69 and > 96 months]. serum cholesterol and triglyceride were measured by enzymatic methods and lipoproteins by precipitation methods. The results were analyzed by LSD and t - test. Regardless of sex and age, the mean values of total cholesterol[TC], total triglyceride[TG], very low density lipoprotein cholesterol[VLDL-c], low density lipoprotein cholesterol[LDL-c], high density lipoprotein cholesterol[HDL-c] were 71.10 +/- 1.25, 26.05 +/- 0.64, 10.91 +/- 0.67, 22.71 +/- 3.74 and 37.61 +/- 1.24 mg/dl, respectively. Statistical analysis showed that there was no difference between female and male ones. However, age had a significant effect on LDL-c concentration so that its value decreased with increase in age. The present study revealed that the normal concentrations of lipids and lipoproteins in Iranian Khouzestanian Arab horses differ from the other breeds and those Arabian horses which live in the other areas. This difference may be due to the effect of breed, nutrition, various physiological and climatic conditions
Subject(s)
Animals , Lipoproteins/blood , HorsesABSTRACT
In order to determine of some blood serum biochemical parameters in rainbow trout, blood samples were taken from the caudal vein of 531 apparently healthy rainbow trout cultured in earthen ponds. Data was analyzes by regression analysis, pearson correlation coefficient test and fisher's test. The mean values of ALP, AST, ALT and LDH were 722 +/- 320, 337 +/- 150, 29 +/- 24 and 579 +/- 353 U/L and the mean values of sodium, potassium and chloride were 144.4 +/- 6.8, 1.8 +/- 0.5 and 126.7 +/- 13.0 mmol/l respectively. The mean values of total protein and albumin were 4.0 +/- 0.7 and 1.7 +/- 0.8 gr/dl. In this study the mean values of uric acid, creatinine, urea, triglyceride, cholesterol, glucose, calcium and phosphorus were 1.7 +/- 0.6, 0.4 +/- 0.2, 9.1 +/- 4.8,273.4 +/- 165.2, 270 +/- 88, 103.8 +/- 35.9, 15.9 +/- 2.9 and 25.3 +/- 5.0 mg/dl respectively. The results of regrisson analysis showed that with increase in age, the mean values of albumin, total protein, cholesterol, sodium, potassium and chloride increased and the mean values of triglyceride, creatinin, AST, ALT and LDH decrease [p<0.05]
ABSTRACT
To compare the ELISA test with digestion method for dignosis sarcocystosis in water buffalo and estimate its sensitivity and specificity. Cross sectional study.Three hundred slaughtered water buffaloes in Ahwaz abattoir.Blood and oesophageal muscles were examined. Oesophageal muscles were examined for sarcocystis by both macroscopic and microscopic [digestion method] examination.Then the ELISA test were designed and compared with digestion method.Finally its sensitivity and specificity were determined. Sensitivity, specificity and 95% confidence intervals were determined by comparing the results obtained by the ELISA assay and digestion method. Me nemar test were used for comparing the percentage of positive cases and their correlation. While specific anti-sarcocytis antibodies were detected in 54.3% of cases macroscpic and microscopic infection were 20% and 57%, respectively. Furthermore, while the positive results of the macroscopic examination significantly differed from those values of the digestion and ELISA methods [P<0.01], no differences were observed between the positive results of the microscopic infection [digestion method] and the ELISA test [P>0.05]. Conclusion: ELISA test providing an effective and reliable means for detecting sarcocystosis in naturally infected water buffaloes. Its simplicity and ease of performance makes it particulary suitable for using in large-scale epidemiological surveys of livestock
ABSTRACT
To investigate the effect of special meterological condition of Ahwaz on thyroid function and to study the effects of gender and age on the function of this gland.Cross sectional study. 210 Sheep.By Considering the meteorologic condition of Ahwaz and biseasonally nature of year in the region, blood samples of 210 sheep were randomly collected during the warmest [February] and coldest [July] months of year from Ahwaz slaughterhouse. Serum T3, T4, T3 uptake and free thyroxin index [FTI] concentrations were measured in four age groups of sheep: less than 6 months, 6-12 months, 12-48 months and above 48 months by Radioimmunoassay. Analysis of variance and Correlation coefficient.There was a significant differences in the concentration of serum T3 [P<0.001], T4 [P<0.001], T3 uptake [P<0.001] and FTI [P<0.05] between the warmest and coldest months. Furthermore, age [but not sex] showed significant effect on these parameters [P<0.05]. Serum T3, T4 and FTI levels was significantly higher and T3 uptake was significantly lower in February than July. A direct correlation between T4 and FTI, T4 and T3, FTI and T3, T3 Uptake and FTI in each month [P<0.01] and a reverse correlation between T3 and T3 uptake [P<0.05] were noticed.The results of this study showed that in July, heat had a suppression effect on thyroid function in sheep. Therefore increased production and reproduction problems in warm season may be due to thyroid dys function. While age showed a negative correlation with thyroid gland function, such pattern did not found for sex
ABSTRACT
To determine concentrations of certain electrolytes in cerebrospinal fluid [CSF] and serum of Khuzestan native buffaloes, samples of CSF from lumbosacral cistern and blood from jugular vein were obtained from 70 clinically-healthy male [n = 30] and female [n = 40], mature [n = 34] and immature [n = 36] animals. Samples were analyzed for sodium and potassium by flame photometry, magnesium by atomic absorption spectrophotometry, calcium by ortocresol phetalein method and phosphorous by phosphomolibdate method using an automated biochemical analyzer. Statistical analyses showed that regardless of age and sex, the mean values of sodium [139.0 +/- 0.70 mmol/1] and magnesium [1.50 +/- 0.24 mmol/1] in CSF were significantly [P<0.001] higher than their mean values in serum; 134.5 +/- 6.3 mmol/1 and 0.72 +/- 0.08 mmol/1, respectively. However, the mean values of potassium [3.47 +/- 0.67 mmol/1], calcium [2.14 +/- 0.25 mmol/1] and phosphorus [1.22 +/- 0.17 mmol/1] in CSF were significantly [P<0.001] lower than their mean values in serum; 5,34 +/- 0.92 mmol/1, 2.33 +/- 0.17 mmol/1 and 1.44 +/- 0.08 mmol/1, respectively. Moreover, in males, females, matures and immature animals, the mean values of sodium and magnesium in CSF were significantly higher than those in the serum and the mean values of potassium, calcium and phosphorus in serum were significantly higher than those in CSF [P<0.01]. Age of animals did not affect any of these electrolytes. The mean value of serum phosphorus in females was significantly higher than that in males [P<0.001]. However, the mean values for potassium, calcium and phosphorus in CSF were significantly higher in males than in females [P<0.05]